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 作者：方显明，袁颖，吴韫宏，尹雪曼 
【关键词】  ,安心颗粒
    ［摘要］  目的：研究安心颗粒对家兔实验性血脂紊乱的防治作用。方法：采用食饵性血脂紊乱新西兰家兔为模型，以脂必妥片为对照药，观察安心颗粒对血脂、主动脉超微结构的影响。将32只新西兰家兔随机分为正常对照组、模型组、脂必妥片组、安心颗粒组等4组，分别给予普通饲料、高脂饲料、高脂饲料加脂必妥片、高脂饲料加安心颗粒。10周后，测定各组动物血清总胆固醇（total cholesterol, TC）、甘油三酯（triglycerides,TG）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol, LDLC）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol, HDLC）、载脂蛋白A1（apolipoprotein A1, ApoA1）、载脂蛋白B（apolipoprotein B, ApoB）水平，同时用电镜观察主动脉超微结构。结果：安心颗粒可以显著降低血脂紊乱新西兰兔血清TC、TG、LDLC、ApoB水平；电镜下主动脉超微结构观察显示：安心颗粒组主动脉硬化病变较模型组和脂必妥片组轻，血管内皮细胞质内偶见小脂滴空泡，各种细胞器存在，内弹力膜存在，中层平滑肌细胞质内无脂滴空泡。结论：安心颗粒能调节兔的血脂代谢，对动脉粥样硬化病变有明显的抑制作用。
　　［关键词］  安心颗粒; 血脂紊乱; 模型, 动物; 主动脉; 超微结构
　　Effects of Anxin Granules on blood lipid and ultrastructure of aorta in rabbits with dyslipidemia
　　　ABSTRACT  Objective: To study the effects of Anxin Granules on dyslipidemia in rabbits caused by high fat plus high cholesterol diet. Methods: Thirtytwo healthy New Zealand male white rabbits were randomly divided into 4 groups: normal control group, untreated group, Zhibituo Tablettreated group and Anxin Granuletreated group. Rabbits in the normal control group were fed with regular chow, while rabbits in the other three groups were fed with high fat plus high cholesterol diet. Zhibituo Tablets and Anxin Granules were administered to the rabbits in Zhibituo Tablettreated group and Anxin Granuletreated group at a daily oral dose respectively. At the end of the 10th week, the levels of serum total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) were tested in each group, and the ultrastructures of the aorta were also observed by an electron microscope. Results: Anxin Granules could reduce the levels of TC, TG, LDLC and ApoB. The results observed by electron microscope showed that, as compared with the untreated group and the Zhibituo Tablettreated group, the atherosclerosis of aorta in the Anxin Granuletreated group was lighter. And it was found that there were few lipid droplet vacuoles in cytoplasm of the endothelial cells, and various cell organs and elastic membrane were existed, but no lipid droplet vacuoles in cytoplasm of the medial smooth muscle cells. Conclusion: Anxin Granules can regulate the metabolism of blood lipid and inhibit the formation of atherosclerosis caused by hyperlipidemia in rabbits.
　　KEY WORDS  Anxin Granules; dyslipidemia; models, animal; aorta; ultrastructure
　　血脂紊乱是由脂质代谢紊乱引起的一种常见病症，也是动脉粥样硬化（atherosclerosis, AS）性心血管疾病最重要的发病因素。因此，有效地调节血脂水平，防治血脂紊乱，防止AS的发生发展，对心血管疾病的防治具有重要意义。安心颗粒是我院治疗冠心病心绞痛取得显著疗效的中药制剂［1］。为了证实该药对血脂紊乱的作用，本课题通过喂饲高脂饲料建立新西兰兔血脂紊乱模型，观察安心颗粒对血脂代谢及动脉粥样硬化病变超微结构的影响，为该药在防治冠心病中的作用提供实验依据。
　　1  材料与方法
　　1.1  实验材料  健康雄性新西兰兔32只，体质量2～2.5 kg，4～5月龄（广西中医学院动物实验中心提供，桂医动字11003）。安心颗粒，由人参、桂枝、栝蒌皮、水蛭、茯苓等药组成，广西中医学院瑞康医院制剂室制备，每包3.5 g（含生药4.5 g/g）。脂必妥片，成都地奥九泓制药厂生产，每片0.3 g，批号20021205。

　　1.2  实验方法
　　1.2.1  模型制作  所有实验动物在实验前均以普通饲料适应性喂饲1周，在稳定其血脂水平及代谢状况后，随机分为4组，即正常对照组、模型组、脂必妥片组和安心颗粒组，每组8只，分笼饲养。正常对照组采用普通饲料喂饲，喂饲10周。其余3组采用高脂饲料喂饲法造模［2］：以1%胆固醇+15%鲜蛋黄+5%猪油+基础饲料为高脂饲料配方，将猪油加热熔化，胆固醇溶于猪油内，另将鲜蛋黄蒸熟后碾碎成粉，猪油、蛋黄粉加到普通饲料中一同搅拌均匀，共喂饲10周。脂必妥片组和安心颗粒组在喂饲高脂饲料的同时，每天分别以脂必妥片、安心颗粒灌胃1次（其中脂必妥片用量为0.156 g・kg1・d1，安心颗粒用量为0.231 g・kg1・d1，其用量按《中药药理实验方法学》中“人和动物按

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